CARL ZEISS LSM 710 (34 CHANNELS)

Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: LSM 710
Nickname: “710”
Software: ZEN black 2012
Year: 2010
SN: 3851001483 (scanmodul SN: 2501000754)

 

 

QUICK GUIDE

Microscope Overview

The Carl Zeiss LSM 710 is a confocal point-scanning microscope able to generate high-resolution three-dimensional images of thick specimens with high sensitivity and low photodamage. It is based on Axio-Observer Z1 inverted microscope body, with motorized XY stage, and has live cell imaging facilities (stage insert for temperature and CO2 control). Applications include for example timelapse imaging, photobleaching experiments, colocalization studies, spectral detection and 3D imaging.

The LSM 710 scanning unit has a spectral recycling loop, and a QUASAR detection unit (34 PMTs). The QUASAR multichannel photomultiplier detection technology is based on a filter-free system that guides the desired wavelength range to the target detector using adjustable optical wedges and slider light stops. The QUASAR detector allow spectral scanning of fluorescence and emission “fingerprinting” as well as linear unmixing of signals from fluorophores with overlapping emission profiles.

System Components

Filter Sets (Locate Mode)

Filter Set

Excitation

Beam Splitter

Emission

Possible Dyes

Set 49

G 365

FT 395

BP 445/50

DAPI, Hoechst

Set 38 (HE)

BP 470/40

FT 495

BP 525/50

FITC, Alexa 488, GFP

Set 43

BP 545/25

FT 570

BP 605/70

Alexa 555, tdTomato, PI

Laser Sets

Laser Unit

Wavelength

Maximum Power

Diode 405-30

405 nm

30 mW

Argon/2

458, 488, 514 nm

25 mW

DPSS 561-10

561 nm

20 mW

HeNe 633

633 nm

5 mW

Objectives

Objective

Magnification

Numerical Aperture

Medium

Observation

Item Number

EC Plan-Neofluar

10x

0.3

air

Ph1, M27

420341-9911-000

Plan-Apochromat

20x

0.8

air

M27

420650-9901-000

Plan-Apochromat

40x

1.4

oil

DIC, M27

420762-9900-000

Plan-Apochromat

63x

1.4

oil

DIC, M27

420782-9900-000

Software

Image acquisition and analysis is performed using the Zen Black 2012 software that includes the following modules:

  • z Stack
  • Time series
  • Tiles and multi-position
  • Autofocus
  • Extended focus
  • Physiology