Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: AxioScan.Z1
Nickname: “Slide Scanner”
Software: ZEN 2.6 (blue)
Year: 2019
SN: 4646000165
Colibri 7 uses up to six different LEDs to generate up to seven fluorescence excitation lines for a sample. All LEDs besides the Green/Yellow line [G/Y] come with individual excitation filters. The [G/Y] line uses a lime LED (567/100 nm) and a motorized filter changer to emit green (555 nm) or yellow (590 nm) light on demand.
Available excitation lines of Colibri 7 are exactly centered around the common dyes and fluorescent protein
Line
Wavelength/Bandwidth
Recommended Dye (Example)
UV
385/30 nm
DAPI, Hoechst 33342, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP/BFP, eGFP (wt), True Blue and similar markers
Violet
423/44 nm
Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean and similar markers
Blue
469/38 nm
Cy2, eGFP, Mitotracker Green, Alexa Fluor 488, Nissl, FITC/Fluorescein, Kaede (green), FuraRed and similar markers
Green
555/30 nm
Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange and similar markers
Yellow
590/27 nm
Mitotracker Red, txRed, mcherry, mRFP1, Rhodamine B, Alexa Fluor 568, Alexa Fluor 594 and similar markers
Red
631/33 nm
Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, Mitotracker DeepRed and similar markers
Far Red
735/40 nm
Alexa Fluor 750, Alexa Fluor 790, Cy7 and similar markers
Excitation
Beam Splitter
Emission
Possible Dyes
FT 420
BP 450/40
DAPI, Hoechst
FT 495
BP 525/50
HE, eGFP
FT 605 (HE)
BP 647/70 (HE)
HE, mRFP
QBS 405+493+575+653
QBP 425/30+514/30+592/25+709/100
HE
TBS 450+538+610
TBP 467/24+555/25+687/145
HE
Objective
Magnification
Numerical Aperture
Medium
Working Distance
Item Number