CARL ZEISS/P.A.L.M. LASER DISSECTING MICROSCOPE

Location: Microscopy Room 2
Manufacturer: Carl Zeiss Microscopy GmbH
Model: P.A.L.M. Laser Dissecting
Nickname: “PALM”
Software: Axiovision & Palm Robot
Year: 2003
SN: 3820005835

Microscope Overview

PALM MicroBeam employs the LMPC method (Laser Microdissection and Pressure Catapulting) to extract biological material of interest out of a tissue specimen. The Zeiss P.A.L.M. system employs a UV laser pulsed microbeam to cut the desired material and the isolated tissue is then recovered by laser pressure catapult into a microfuge tube cap. Cutting accuracy of less than 1 µm can be achieved.
The source material can be placed on a normal objective glass slide or on a membrane slide, depending on the way of approaching. The system can be used with frozen or paraffin-embedded tissue samples, single cells, and even single chromosomes.

 

For more information:
http://microscopy.zeiss.com/microscopy/en_de/products/laser-microdissection/microbeam.html

Webinars:
http://www.zeiss.com/microscopy/en_de/products/laser-microdissection/microbeam.html – webinars

System Components

Objectives

Objective

Magnification

Numerical Aperture

Medium

Observation

Item Number

5x

0.25

air

For microdissection

440125-0000-000

20x

0.4

air

For microdissection
Correction Ring
(Coverglass thickness 0-1.5 mm)

441340-9970-000

40x

1.3

air

For microdissection
Correction Ring
(Coverglass thickness 0-1.5 mm)

441350-9970-000

100x

1.3

oil

Ph3

000000-1018-595

Software

Software (the system contains two different software, which CANNOT be operated simultaneously):
Axiovision Software (for Imaging Analysis):

   Modules

  • z Stack
  • Time Lapse
  • MosaiX
  • Multichannel
  • Extended Focus
  • Automeasure Plus
  • Commander

 

PALM Robot Software (for Microdissection):

   Modules

  • Autofocus
  • LMPC-Module
  • Extended Focus
  • Multichannel Fluorescence