Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.

Therefore, the Cell Observer Spinning Disk (SD) is a live cell imaging platform for high-speed confocal imaging using the CSU-X1M spinning disc technology from Yokogawa and one highly sensitive electron multiplying camera (EM-CCD Evolve Delta). This system is based on a motorized Carl Zeiss Axio Observer Z1 inverted scope with Definite Focus.2, multi-channel acquisition, piezo x/y/z stage, DirectFRAP unit, and a full environmental chamber for temperature and atmosphere control.

The Definite Focus.2 system allows for long-term time-lapse experiments without any focus drifts. In addition, the DirectFRAP is the ideal solution for any type of laser manipulation in the living cell – FRAP, FLIP, photoactivation or conversion.

Almost the entire microscope, from filter wheels to sample incubation parameters, are computer-controlled via the Zen Blue 3.2 software.

– Microscope: Zeiss Axio Observer
– Confocal scanner: Yokogawa CSU-x1
– EMCCD Camera: Evolve 512 EMCCD
– Definite Focus.2
              | Introduction to Definite Focus.2
              | Perform Large Tile Experiments with Definite Focus.2
              | Multi-Position Experiments with Definite Focus.2
              | Multi-Position Time Series with Definite Focus.2