CARL ZEISS CELL OBSERVER SPINNING DISK

Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: Cell Observer SD
Nickname: “Spinning Disk”
Software: ZEN 3.2 (Blue Edition)
Year: 2012
SN: 3851001529

Microscope Overview

Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and photosensitive processes in living cells.

The Cell Observer Spinning Disk (SD) is a live cell imaging platform for high-speed confocal imaging using the CSU-X1M spinning disk technology from Yokogawa and a highly sensitive electron multiplying camera (EM-CCD Evolve Delta). The system is based on a motorized Carl Zeiss Axio Observer Z1 inverted microscope with Definite Focus.2, multi-channel acquisition, piezo x/y/z stage, DirectFRAP unit, and a full environmental chamber for temperature and atmosphere control.

The Definite Focus.2 allows hardware focus control during long time-lapse imaging to prevent focus drift. In addition, the DirectFRAP is the ideal solution for any type of laser manipulation in the living cell – FRAP, FLIP, photoactivation or conversion.

Almost the entire microscope, from filter wheels to sample incubation parameters, is computer-controlled via the Zen Blue 3.2 software.


– Confocal scanner: Yokogawa CSU-x1
EMCCD Camera: Evolve 512 EMCCD (512 × 512 pixels, pixel size: 16 × 16 µm)
Definite Focus.2
              | Introduction to Definite Focus.2
              | Perform Large Tile Experiments with Definite Focus.2
              | Multi-Position Experiments with Definite Focus.2
              | Multi-Position Time Series with Definite Focus.2

System Components

Objectives

Objective

Magnification

Numerical Aperture

Medium

Observation

Item Number

20x

0.8

air

M27

420650-9901-000

40x

1.3

oil

DIC, M27

420462-9900-000

63x

1.3

water or glycerol

DIC, M27

420882-9971-000

100x

1.46

oil

DIC, M27

420792-9800-000

Filter Sets (Locate Mode)

Filter Set

Excitation

Beam Splitter

Emission

Possible Dyes

G 365

FT 395

BP 445/50

DAPI, Hoechst

BP 470/40

FT 495

BP 525/50

FITC, Alexa 488, GFP

BP 545/25

FT 570

BP 605/70

Alexa 568, mcherry, PI

Laser Sets (confocal)

Laser Unit

Wavelength

Maximum Power

Solid State Laser 405

405 nm

50 mW

Solid State Laser 488

488 nm

100 mW

Solid State Laser 561

561 nm

50 mW

Emission Filters (confocal)

Laser Unit

Transmission

Emission Dyes

1. BP 450/50

425-475 nm

     

2. BP 485/30

470-500 nm

3. DBP 480/22 + LP530

469-491 nm + >530 nm

   

4. DBP 527/54 + 645/60

500-554 nm + 615-675 nm

  

5. BP 562/45

540-585 nm

6. BP 629/62

598-660 nm

Software

Image acquisition and analysis is performed using the Zen 3.2 (Blue) software that includes the following modules:

  • z Stack
  • Time series
  • Tiles and multi-position
  • Autofocus
  • Extended focus