CARL ZEISS AXIO IMAGER.Z2

Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: AxioImager.Z2
Nickname: “Imager” or “Z2”
Software: ZEN 3.2 (blue)
Year: 2010
SN: 3523000868

QUICK GUIDE

Microscope Overview

The Axio Imager Z2 system is a fully motorized upright widefield microscope, which is ideal for quantitative analysis. Moreover, the system can perform structured illumination acquisition by means of ApoTome2.
The system is equipped with a CCD monochromatic digital camera (Axiocam HRm) and a CCD color digital camera (Axiocam HRc).
Stereological studies are also possible using StereoInvestigator software.
The ZEN Blue 3.2 software fully controls the microscope and the cameras, allowing the acquisition of multidimensional images (multi-channel, time series, z-stack, tiles or multi-positions).

System Components

Filter Sets

Filter Set

Excitation

Beam Splitter

Emission

Possible Dyes

Set 49

G 365

FT 395

BP 445/50

DAPI, Hoechst

Set 38 (HE)

BP 470/40

FT 495

BP 525/50

FITC, Alexa 488, GFP

Set 31

BP 565/30

FT 585

BP 620/60

Alexa 568, mcherry

Set 50

BP 640/30

FT 660

BP 690/50

Alexa 667, Cy5

Objectives

Objective

Magnification

Numerical Aperture

Medium

Observation

Item Number

EC Plan-Neofluar

5x

0.16

air

M27

420330-9901-000

EC Plan-Neofluar

10x

0.3

air

M27

420340-9901-000

Plan-Apochromat

20x

0.8

air

M27

420650-9901-000

Plan-Apochromat

40x

1.3

oil

DIC, M27

420762-9800-000

Plan-Apochromat

63x

1.4

oil

DIC motorized, M27

420782-9900-000

Plan-Apochromat

100x

1.4

oil

Ph3, M27

420791-9911-000

Software

The acquisition and analysis of images is performed on the Zen Blue 3.2 software.
The software has the following modules:

  • z Stack
  • Tiles and multi-position
  • Multichannel
  • Autofocus
  • Experiment Manager
  • ApoTome2

 

For stereology analysis, the system has the StereoInvestigator software.
The Software has the following modules:
 
  • Stack