Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: AxioScan.Z1
Nickname: “Slide Scanner”
Software: ZEN 2.6 (blue)
Year: 2019
SN: 4646000165
The Zeiss Axio Scan.Z1 slide scanner for brightfield and fluorescence delivers exceptional image quality, making it ideal for even the most demanding virtual microscopy applications. This advanced system can scan up to 100 slides in a single run and features a fully motorized condenser, LED illumination, and highly sensitive color and monochromatic cameras. These capabilities enable high-speed digitization while maintaining superior image quality.
– LED Illumination: Zeiss Colibri 7
– Digital Cameras:
| Camera (for brightfield): Hitachi HV-F203SCL (1920 × 1200 pixels; pixel size: 3.45 × 3.45 µm)
| Camera (for fluorescence): Orca Flash 4.0 V3 (2048 × 2048 pixels; pixel size: 6.5 × 6.5 µm)
Colibri 7 uses up to six different LEDs to generate up to seven fluorescence excitation lines for a sample. All LEDs besides the Green/Yellow line [G/Y] come with individual excitation filters. The [G/Y] line uses a lime LED (567/100 nm) and a motorized filter changer to emit green (555 nm) or yellow (590 nm) light on demand.
Available excitation lines of Colibri 7 are exactly centered around the common dyes and fluorescent protein
Line
Wavelength/Bandwidth
Recommended Dye (Example)
UV
385/30 nm
DAPI, Hoechst 33342, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP/BFP, eGFP (wt), True Blue and similar markers
Violet
423/44 nm
Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean and similar markers
Blue
469/38 nm
Cy2, eGFP, Mitotracker Green, Alexa Fluor 488, Nissl, FITC/Fluorescein, Kaede (green), FuraRed and similar markers
Green
555/30 nm
Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange and similar markers
Yellow
590/27 nm
Mitotracker Red, txRed, mcherry, mRFP1, Rhodamine B, Alexa Fluor 568, Alexa Fluor 594 and similar markers
Red
631/33 nm
Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, Mitotracker DeepRed and similar markers
Far Red
735/40 nm
Alexa Fluor 750, Alexa Fluor 790, Cy7 and similar markers
Objective
Magnification
Numerical Aperture
Medium
Working Distance (mm)
Item Number
Position
Beam Splitter
1
None
2
FT 495
3
FT 420
4
FT 605
5
QBS 405+493+575+653
6
TBS 450+538+610
Position
Beam Splitter
1
None
2
BP 525/50
3
BP 450/40
4
BP 647/70
5
QBP 425/30+514/30+592/25+709/100
6
TBP 467/24+555/25+687/14