Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: AxioImager.Z2
Nickname: “Imager” or “Z2”
Software: ZEN 3.2 (blue)
Year: 2010
SN: 3523000868
The Axio Imager Z2 system is a fully motorized upright widefield microscope that provides fluorescence, brightfield and DIC (Differential Interference Contrast) multichannel imaging. Additionally, the system allows optical sectioning imaging based on structured illumination acquisition by means of ApoTome2.
The system is equipped with a CCD monochromatic digital camera (Axiocam HRm), for fluorescence imaging, and a CCD color digital camera (Axiocam HRc), for brightfield imaging. The ZEN Blue 3.2 software fully controls the motorized microscope and the cameras, enabling automated multichannel, tiled and optical section imaging.
Stereological studies are also possible using Stereo Investigator software.
The ApoTome2 uses structured illumination to remove out-of-focus light during imaging. By projecting a moving grid structure into the focal plane of your specimen along with an automated scanning mechanism, the Apotome 3 automatically acquires and processes a series of digital images into one optical section with improved contrast and increased resolution.
Conventional Fluorescence
Apotome
Stereo Investigator is a software package developed to help to make the most accurate, unbiased quantification of the number, length, area, and volume of cells, subcellular and macro structures in your tissue specimens.
If you wish to learn more, visit the MBF Bioscience comprehensive stereology information site, stereology.info or watch the YouTube webinars.
– Microscope Stand: Zeiss Axio Imager.Z2
– Digital Cameras:
| Axiocam HRm – CCD digital camera (1300 × 1030 pixels; pixel size: 6.7 × 6.7 µm)
| Axiocam HRc – CCD digital camera (1300 × 1030 pixels; pixel size: 6.7 × 6.7 µm)
Filter Set
Excitation
Beam Splitter
Emission
Possible Dyes
Objective
Magnification
Numerical Aperture
Medium
Observation
Item Number
The acquisition and analysis of images is performed on the Zen Blue 3.2 software.
The software has the following modules:
For stereology analysis, the system has the StereoInvestigator software.