CARL ZEISS AXIO OBSERVER.Z1

Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: AxioObserver.Z1
Nickname: “Observer”
Software: ZEN 3.2 (blue)
Year: 2010
SN: 3834004066

Microscope Overview

The Axio Observer Z1 system is a fully motorized inverted widefield microscope, which is ideal for live imaging experiments. It is equipped with a large stage incubator for temperature and humidity control and a stage CO2 incubator.
The system is equipped with a CCD digital camera (Axiocam HRm), a digital CMOS camera (ORCA Flash 4.0) and a Zeiss axiocam 305 color.
The ZEN Blue 3.2 software allows to fully control the microscope and the cameras, allowing the acquisition of multidimensional images (multi-channel, time series, z-stack, tiles or multi-positions).

System Components

Filter Sets

Filter Set

Excitation

Beam Splitter

Emission

Possible Dyes

G 365

FT 395

BP 445/50

DAPI, Hoechst

BP 470/40

FT 495

BP 525/50

FITC, Alexa 488, GFP

BP 565/30

FT 585

BP 620/60

Alexa 568, mcherry

BP 640/30

FT 660

BP 690/50

Alexa 667, Cy5

Set

BP 400-450

BS 485

BP 635-675

QD 655

Objectives

Objective

Magnification

Numerical Aperture

Medium

Observation

Item Number

10x

0.3

air

Ph1, M27

420341-9911-000

20x

0.8

air

M27

420650-9901-000

20x

0.4

air

Ph2, M27

421351-9972-000

40x

0.95

air

Correction Ring (Korr)

(Coverglass thickness 0.13-0.21mm)

420660-9970-000

40x

1.3

oil

DIC

440452-9903-000

63x

1.4

oil

DIC, M27

420782-9900-000

Software

The acquisition and analysis of images is performed on the Zen Blue 3.2 software.
The software has the following modules:

  • z Stack
  • Time series
  • Tiles and multi-position
  • Multichannel
  • Software Autofocus
  • Extended focus
  • Experiment Manager
  • Measurements
  • Advanced Processing