Location: Microscopy Room
Manufacturer: Carl Zeiss Microscopy GmbH
Model: Cell Observer SD
Nickname: “Spinning Disk”
Software: ZEN 3.2 (Blue Edition)
Year: 2012
SN: 3851001529
Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and photosensitive processes in living cells.
The Cell Observer Spinning Disk (SD) is a live cell imaging platform for high-speed confocal imaging using the CSU-X1M spinning disk technology from Yokogawa and a highly sensitive electron multiplying camera (EM-CCD Evolve Delta). The system is based on a motorized Carl Zeiss Axio Observer Z1 inverted microscope with Definite Focus.2, multi-channel acquisition, piezo x/y/z stage, DirectFRAP unit, and a full environmental chamber for temperature and atmosphere control.
The Definite Focus.2 allows hardware focus control during long time-lapse imaging to prevent focus drift. In addition, the DirectFRAP is the ideal solution for any type of laser manipulation in the living cell – FRAP, FLIP, photoactivation or conversion.
Almost the entire microscope, from filter wheels to sample incubation parameters, is computer-controlled via the Zen Blue 3.2 software.
– Confocal scanner: Yokogawa CSU-x1
– EMCCD Camera: Evolve 512 EMCCD (512 × 512 pixels, pixel size: 16 × 16 µm)
– Definite Focus.2
| Introduction to Definite Focus.2
| Perform Large Tile Experiments with Definite Focus.2
| Multi-Position Experiments with Definite Focus.2
| Multi-Position Time Series with Definite Focus.2
Objective
Magnification
Numerical Aperture
Medium
Observation
Item Number
Filter Set
Excitation
Beam Splitter
Emission
Possible Dyes
Laser Unit
Wavelength
Maximum Power
Solid State Laser 405
405 nm
50 mW
Solid State Laser 488
488 nm
100 mW
Solid State Laser 561
561 nm
50 mW
Laser Unit
Transmission
Emission Dyes
1. BP 450/50
425-475 nm
2. BP 485/30
470-500 nm
3. DBP 480/22 + LP530
469-491 nm + >530 nm
4. DBP 527/54 + 645/60
500-554 nm + 615-675 nm
5. BP 562/45
540-585 nm
6. BP 629/62
598-660 nm
Image acquisition and analysis is performed using the Zen 3.2 (Blue) software that includes the following modules: